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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 9 Documents

Search results for , issue "Vol 25 No 2 (2020): June 2020" : 9 Documents clear

Phytochemical properties and antioxidant activities of the leaf extracts of Boenninghausenia albiflora (Hook.) Rchb. ex. Meisn. (Rutaceae) I Putu Agus Hendra Wibawa; Putri Sri Andila; Vienna Saraswaty; I Gede Tirta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Boenninghausenia albiflora is a well-known plant for being used in various traditional treatments. The plant is also reported to contain an essential oil which has a distinctive and pungent aroma. The present study aimed at investigating extraction yields, phytochemical compounds and antioxidants, and potential uses of B. albiflora leaves extracted with acetone solvent. Bioactive compounds of the leaf extract of B. albiflora were identified by GC-MS analysis while the antioxidant assay was measured by DPPH method. The extract obtained from acetone contained 30 compounds including coumarin group, 2-Isoprenyl-2,3-dihydro-furo [3,2-G] chromen-7-one (22.89% w/v) as the highest percentage compound. Other coumarins such as suberosin and dehydrogeijerin were also identified in this study. The IC50 values of the leaves extract of B. albiflora was 194.3 ppm which was higher than propyl gallate. Thus indicating B. albiflora as potential sources of anti-cancer, anti-inflammatory and antioxidant.

Combination of culture and dyeing conditions on cloth color dyed with mixed fungi dyes Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

This study aimed to examine the dyes production of mixed Aspergillus and Paecilomyces on different culture conditions and evaluate the combination between culture and dyeing conditions on cloth color by the fungal dyes. The different media consist of mineral salt glucose (MSG) medium and mineral salt sucrose (MSS) medium were evaluated. Additionally, different culture conditions such as C and N sources, incubation temperature, and pH of the media were evaluated together with variation of dyeing conditions including pH and mordants. The result showed that different culture conditions on media and the combination of those with dyeing conditions produce distinction colors on the fabric. The variation of dyed colors on the fabric was related to the dyes concentration produced by combination treatment of culture condition and dyeing pH. However, the effect was not observed on the combination between culture condition and mordants. Combination of culture and dyeing conditions on cloth color dyed with mixed fungi dyes generated various cloth colors, adding a color variation on textile dyeing.

In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer Sutini Sutini; Widiwurjani Widiwurjani; Nora Augustien; Hadi Suhardjono; Guniarti Guniarti; Djoko Agus Purwanto; Wirdhatul Muslihatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.

An evaluation on cropping system of rice and corn in dry land during dry season Zainal Arifin; Amik Krismawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Dry land has wide and diverse agroecosystem, which potential as growth source to produce dietary crops (foodstuff). The objective of the research was to study the appropriate managerial technique of cropping system for rice and corn to increase production and income of the farm operation in dry land during dry season. The research was conducted on dry land during dry season (DS) I in 2018 at Jatisari Village, Arjasa Subdistrict, Situbondo Regency, on the area of 10,000 m2 width. The research used Randomized Block Design with 4 treatments and 6 replications, includes : (a) Upland rice (20 cm x 20 cm), (b) Upland rice largo super (40 cm x 20 cm x 10 cm), (c) Upland rice largo super (40 cm x 20 cm x 10 cm) + corn (160 cm x 40 cm), and (d) Corn (75 cm x 20 cm). Results of the research showed that the intercropping system between upland rice largo super and corn produced higher yield, 8,151 kg/ha DGH and 2,092 kg/ha grains of corn. Besides that, yield of corn trimming was 2,446 kg/ha fresh biomass. The increase production of intercropping upland rice largo super with corn has increase income of the farmers, IDR. 41,923,100 with R/C ratio 3.25, so that it is economically feasible.

In vitro embryogenic callus induction of Carica pubescens Lenne and K.Koch using 2,4-D (2,4-Dichlorophenoxy Acetic Acid) and BAP (6-Benzylaminopurin) Shinta Shinta; Eko Budi Minarno; I’anatur Rofiqoh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Carica pubescens Lenne & K. Koch has benefits as a producer of medicinal compounds. Propagation of C. pubescens is important to be done through in vitro embryogenic callus culture. This research aimed to determine the effect of 2,4-D growth regulators, 2,4-D and BAP interactions on the induction of embryogenic callus C. pubescens Lenne & K. Koch. This research is experimental, using a completely randomized design (CRD) with 25 treatments and 5 replications. Embryonic callus was induced by various concentration of 2,4 D (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L) in combination with BAP (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L). Data were analyzed using two-way ANOVA, followed by the Duncan Multiple Range Test (DMRT) (p>0.05). The result showed that the addition of 2,4 D and BAP as a growth regulator gave an effect on embryonic callus. Concentration of 7.5 mg/L 2,4 D and 0.5 mg/L BAP in callus 19 days after planting showed the most effective interaction of both inductors. The combination produced callus fresh weight at 1.09 g and 99% in percentage of explant callus area. Morphological and anatomical observations showed the characteristic of embryogenic callus as indicated by yellowish, textured callus friable, having a large core and containing starch grains also plastids.

Virtual prediction of antiviral potential of ginger (Zingiber officinale) bioactive compounds against spike and MPro of SARS-CoV2 protein Ahmad Hafidul Ahkam; Feri Eko Hermanto; Adzral Alamsyah; Iva Himmatul Aliyyah; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Coronavirus disease 2019 (COVID-19) is a human disease caused by SARS-CoV2 becomes a serious health threat after infected more than 6 million people globally. The virus enters the host cell through an S protein on its surface and begins its life cycle with the help of a key protein, MPro. On the other hand, several bioactive from Ginger have been reported for their antiviral properties, but few studies related to COVID-19. This study aims to evaluate the potential of a few bioactive compounds from Ginger as anti-SARS-CoV2. Gingerenone A, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone were used as ligand to be docked with S protein and MPro. Drug-likeness properties also evaluated using SwissADME. Gingerenone A constantly gave the lowest binding energy compared to others both with S or MPro. However, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone could interact with key residues responsible for MPro catalytic domain, while geraniol, shogaol, zingiberene, zingiberenol, and zingerone could interfere S-ACE2 binding shape and increase its binding energy. The drug-likeness analysis also revealed that all of the analyzed compounds have no violation of Lipinski’s Rule of 5. In conclusion, gingerol, geraniol, shogaol, zingiberene, zingiberenol, and zingerone from Ginger have good potential as antiviral agents with good oral bioavailability and flexibility

Future zoonis forecasting of Betacoronavirus group using phylogenetics and haplotype network analysis Rahmat Grahadi; Rizka Vamelia Sulistya Ningrum; Najma Zahira; Nia Kurniawan; Fatchiyah Fatchiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Genetic diversity and species relationship contribute to the inter-species transmission of coronavirus-derived diseases. This research aimed to investigate the probability of newly emergence Betacoronavirus zoonosis according to genetic relationship and haplotype network. A total of 24 Betacoronavirus sequences from different continents were used to construct the phylogenetic tree and the haplotype network. A phylogenetic tree was constructed using the Maximum Likelihood method with RaxML in CIPRES Science Gateway portal and GTRGAMMA+I analysis with 1000 bootstrap. Haplotype Network was done using Network 10.0.0.0 version with median-joining analysis. The result of the phylogenetic and haplotype network formed 4 groups of Betacoronavirus. Group A consisted of Human Coronavirus (HCoV) type OC43 and HKU1, Bovine Coronavirus, and Rodent Coronavirus. Group B consisted of SARS-CoV and SARS-CoV-2. Group C consisted of MERS-CoV (2012 pandemic) and MERS-CoV from camels. Reliable with phylogenetic results, haplotype network also grouped Betacoronaviruses into three groups in accordance with their subgenera. Bovine and Rodent coronavirus are constant to group with previously human coronavirus, i.e. HCoV-OC43 and HCoV-HKU1, respectively. According to the high genetic similarity that the Bovine and Rodent coronavirus may infect to human and provide a new emergence. This study is basic for further research related to inter-species transmission from animal to human.

The UV-Vis spectrum of antioxidant complex from frozen-dried egg white protein and tomato extract Siti Imama Khoiriyah; Mohammad Mabrur; Sutiman Bambang Sumitro; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value was lower than the single ingredient, while the tomato extract was higher than the complex profile. On the contrary, the NFD complex showed a higher peak absorption value than to every single ingredient. Based on each UV-Vis spectrum profile, the tomato extract profile shifted the absorption peak, and the absorbance value of the FD sample was changing higher than the NFD sample, while the egg white protein spectrum profile did not change significantly in the FD and NFD samples. The absorption peak of the complex is higher than the single ingredient indicated that the characterization of the complex had been formed. In the NFD complex profile, the absorption peaks formed at the wavelength regions of 230 nm and 280 nm with absorbance values of 2.51 and 0.76 and its number has higher than those of the FD complex with absorbance values of 2.47 and 1.82 at wavelengths of 220 and 265 nm.

Tissue repair in post extraction sockets of Cavia cobaya induced by a combination of propolis and graft Utari Kresnoadi; Roselini Halim; Hananah Oktalidial Putri; Mutiara Aryanita; Imam Safari Azhar
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Propolis, hive defensive substance that contains caffeic acid phenethyl ester, can affect the migration and proliferation of cells involved in the healing process. Combination of propolis extract with BBG are predicted to fasten the wound healing, especially its effect on angiogenesis, macrophages and collagen density. In this study, we aimed to analyze the effect of a propolis combination and BBG in proliferating new blood vesselsand macrophages, while also increasing the collagen density of the post-extracted tooth socket healing process in Cavia cobaya. 56 C. cobaya, whose left mandibular incisorshad been extracted, were subsequently divided into four groups on the basis of the substances used to fill their sockets, namely; control, BBG, propolis extract, and a combination of BBG, and propolis extract. The subjects sacrificed on either the third or seventh day. Histopathological samples were produced using HE and MT staining. The results were subjected tosome statistical analysis tests: one-way analysis of variance (ANOVA), Tukey’s honestly significant difference (HSD), Kruskal Wallis, and Mann Whitney. Significant increases (p<0.05) were detected between the PEG and PEG+Propolis groups and also between the PEG and PEG+BBG+Propolis combination groups. However, an insignificant increase (p>0.05) was identified between the PEG and BBG groups. In conclusion, a combination of BBG and propolis can increase the number of new blood vessels and macrophages together with the degree of collagen density in the healing process of post-extraction tooth sockets of C. cobaya.

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

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+62341570631

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wulidanisa@berkalahayati.org

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Jalan Surakarta No. 5 Malang, Indonesia

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA